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Compared to FACS, our GST affinity cell sorting system does not require expensive equipment and is less likely to cause mechanical cell damage ( The recently reported positive cell sorting system utilizes GPI-anchored Twin-Strep-Tag as the affinity tag and allows the positive cells to be enriched with Strep-Tactin magnetic beads ( Reduced GSH is widely expressed in plant, animal, and fungal cells ( In brief, we develop a gene transfection-positive cell sorting system utilizing GPI-anchored GST as the affinity tag that enables positive cell isolation using GSH magnetic beads

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A 20–30 μg of total protein were resolved on 4–12% NuPAGE Bis–Tris gels (Thermofisher) and transferred to nitrocellulose membrane using a semi-dry iBlot2 kit (Thermofisher)