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To be able to measure the production of ROS, studies often use flow cytometry in combination with fluorescence or chemiluminescent assays to detect levels of commonly produced ROS, such as hydrogen peroxide, a hydroxyl radical, and a peroxynitrite anion [60]

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In HCT-116 cells (Marimuthu et al, 2011), aspirin acetylated at least 33 different proteins, which included histones, cytoskeletal and heat shock proteins, glycolytic and pentose phosphate pathway enzymes and ribosomal and mitochondrial proteins